A simple and accurate HPTLC method has been developed for determination of Glucosamine and Boswellia serrata extract in their tablet dosage form. The method employed HPTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. Toluene: Ethylacetate (2.5:1.5 v/v) as a mobile phase-A and N-Butanol: Water: Glacial acetic acid (3:1:1 v/v/v) as a mobile phase-B. After that saturation time is optimized for the better separation. The Rf value of Glucosamine, 11-Keto-β-Boswellic acid and Acetyl-11-Keto-β-Boswellic acid are 0.33±0.02, 0.43±0.02 and 0.51±0.02 respectively were found. The linearity for GLU, 11-KBA and A-11-KBA were in the range of 6250-31250, 300-1500 and 150-750 ng/band respectively. The % recoveries of GLU, 11-KBA and A-11-KBA were found to be in the range of 99.93-99.96, 99.06-99.40 and 98.22-99.36% respectively. The proposed method was validated and successfully applied for the estimation of GLU, 11-KBA and A-11-KBA in combined tablet dosage form.
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